KCBD 1250 and Abbott 129

    Announcements


    Core_Fair_October_31_2017_11am-2pm

    Olympus FV3000 Confocal Demo October 10th-19th

    FV3000_demo_flyer

    ImageJ Classes Fall Quarter 2017

    Fall Quarter ImageJ classes are up! See class descriptions here. Please register for a class and/or workshop on our ImageJ help page.

    ImageJ Basics Thursday Oct. 5th 1:30-3:00pm Cummings 119
    Intermediate ImageJ Thursday Oct. 12th 1:30-3:00pm Cummings 119
    Macro Writing Workshop Thursday Oct. 26th 1:30-3:30pm Cummings 119

    LED Lightsources Added to Spinning Disk Confocals

    August 25, 2017 - Thanks to University funds the Core is going GREEN! We've installed two energy-efficient, long-lasting SOLA SE 365 FISH LED light engines from Lumencore to our existing Nipkow spinning disk confocals (the live and fixed DSUs).

    3 color 3D STED Upgrade Complete!

    July 24, 2017 - The Leica SP8 laser scanning confocal has been upgraded! The University has given us the funds to add 3-color, 3D gated Stimultated Emission Depletion (STED) and real-time deconvolution (HyVolution) to our existing Leica SP8. Contact Christine or Vytas for more information and training!

    ImageJ Classes Summer Quarter 2017

    Summer Quarter ImageJ classes are up! Summer Sessions combine Basics and Intermediate classes (See class descriptions here) into one long session so you only need to attend one day. Please register for a class and/or workshop on our ImageJ help page.

    Summer Session #1 Wednesday June 14th 1:00-3:30pm Cummings 119
    Summer Session #2 Wednesday June 21st 1:00-3:30pm Cummings 119
    Macro Writing Workshop Wednesday June 28th 1:30-3:30pm Cummings 119

    Image Cytometry Seminar January 20, 2017 10-11:30am KCBD 1103

    Join us for a seminar on high-throughput screening with the Celigo Image Cytometer from Nexcelom Bioscience

    Celigo_Flyer

    ImageJ Classes Winter Quarter 2017

    Winter Quarter ImageJ classes are up! (See class descriptions here). Please register for a class and/or workshop on our ImageJ help page.

    ImageJ Basics Wednesday January 25th 2:00-3:00pm Cummings 119
    Intermediate ImageJ Wednesday February 1st 2:00-3:00pm Cummings 119
    Macro Writing Workshop Wednesday February 8th 1:30-3:30pm Cummings 119

    Important message for chamber slide users!

    Universal stages can be found on the Leica SP8, Leica SP5 STED, Leica SP5 2-photon, Marianas, Olympus IX81 and Olympus live prep DSU

    Chamber_slide_slgn

    Join us for Core Fair 2016! Wednesday October 19th 11am-2pm in the GCIS 3rd Floor Atrium

    Core_Fair_Flyer

    ImageJ Classes Fall Quarter 2016

    Christine's ImageJ classes are back for Fall Quarter! There will be one session each of Basics, Intermediates and the Macro Writing Workshop (see class descriptions here). Please register for a class and/or workshop on our ImageJ help page.

    ImageJ Basics Tuesday October 4th 2-3:30pm Cummings 119
    Intermediate ImageJ Tuesday October 11th 2:00-3:30pm Cummings 119
    Macro Writing Workshop Tuesday October 18th 2:00-4:00pm Cummings 119


    Product Demo - Upgraded Caliber I.D. RS-G4 Large Format Confocal Microscope

    RS-G4
    July 2016 - Caliber I.D. has upgraded our large format laser scanning confocal demo to FIVE lasers (405nm, 488nm, 561nm, 638nm and 785nm)!

    The RS-G4 is an upright confocal microscope with a motorized XY-Z stage and software designed for large format images. This system is ideal for high speed, high quality tiling in 2D or 3D and will handle samples up to 120mm x 80mm x 6mm thick.

    Data is output in multi-tiff format for ease of use in your favorite image processing software (ImageJ, Photoshop, etc). To set up a time to see this exciting new microscope and scan some slides, contact Shirley Bond (info at left).


    ImageJ Summer Sessions 2016

    ImageJ Summer Sessions combine ImageJ Basics and Intermediate ImageJ ( see class descriptions) into one super-sized class. Christine has scheduled two sessions of this class, both present the same material. Please register for a class and/or workshop on our ImageJ help page.

    Tuesday June 21st 2:30-5:00pm Cummings 119
    Wednesday July 13th 2:00-4:30pm Cummings 119
    She will also hold a Macro Writing Workshop on Wednesday July 20th 2:00-4:00pm in Cummings 119


    New Equipment - Lattice Lightsheet Bessel Beam Illumination Microscope

    LatticeLightsheet
    This is a clone of the Betzig system running at Janelia Farm, first described in Science October 2014. It is designed for low-light 3D time lapse imaging. It includes 405, 488, 561, and 642nm laser lines and a Hamamatsu Flash4v2+ sCMOS imaging camera. This is NOT like the Zeiss Lightsheet.z1 meant for large preps! Imaging is by means of water-immersion objectives and is limited to less than 100 micrometers depth of penetration. The imaging lens has NA 1.1 and yields 100nm pixels. All samples must be on 5mm round coverslips. Use of this microscope requires a Staff person to operate the system! Please be sure to arrange your imaging session with Core Staff BEFORE you start sample prep.





    Imaris Workshop Friday June 3, 2016

    Imaris 8.2

    This Friday the Core will host Amanda Howard, PhD of Bitplane for an all-day Imaris workshop. The day will start with a lecture on all the capabilities of Imaris 8.3 (KCBD 1280 9:30-10:30am) followed by one-on-one data processing sessions with Dr. Howard on the new high-end Imaris workstation. Workshop sessions are free but by appointment only, please contact Vytas, Christine or Shirley to book.



    Leica SP8 is Ready for Imaging!

    SP8
    The new Leica SP8 is up and running! See our equipment page for specs and FAQ about the SP8. Contact Christine (info at left) to set up your training session. All users will need to be trained for the SP8 regardless of past experience with the SP2 or SP5s.




    Product Demo - Caliber I.D. RS-G4 Large Format Confocal Microscope

    RS-G4
    February 2016 - this month we have a brand new microscope on demo from Caliber I.D.. The RS-G4 is an upright confocal microscope with a motorized XY-Z stage and software designed for large format images. This system is ideal for high speed, high quality tiling in 2D or 3D and will handle samples up to 120mm x 80mm x 6mm thick.

    On the demo model we have lasers for 488nm (greens like GFP, CY2, and Alexa 488), 638 (deep reds like APC, Cy5 and Alexa 647) and 785 (near IR dyes like Cy7 or Alexa 750). Data is output in multi-tiff format for ease of use in your favorite image processing software (ImageJ, Photoshop, etc). To set up a time to see this exciting new microscope, contact Shirley Bond or one of the other Technical Directors (info at left).


    Spring Quarter ImageJ Classes

    Our room size is limited so please register by emailing Christine at ccase@uchicago.edu. Descriptions of all of the classes can be found on our ImageJ help page.

    ImageJ Basics - Wednesday April 6, 2016 2:00-3:30pm Cummings 119 -- OPEN

    Intermediate ImageJ - Wednesday April 13, 2016 2:00-3:30pm Cummings 119 -- OPEN

    Macro Writing Workshop - Thursday April 21, 2016 2:00-4:00pm Cummings 119 -- OPEN

    Macro Writing Workshop - Wednesday April 27, 2016 2:00-4:00pm Cummings 119 -- OPEN


    Winter Quarter ImageJ Classes

    Our room size is limited so please register by emailing Christine at ccase@uchicago.edu. Descriptions of all of the classes can be found on our ImageJ help page.

    ImageJ Basics - Wednesday February 10, 2016 2:00-3:30pm Cummings 119 -- DONE!

    Intermediate ImageJ - Wednesday February 17, 2016 2:00-3:30pm Cummings 119 -- DONE!

    Macro Writing Workshop - Wednesday February 24, 2016 2:00-4:00pm Cummings 119 -- DONE

    Macro Writing Workshop - Wednesday March 2, 2016 2:00-4:00pm Cummings 119 -- LIMITED


    Imaris 8.2 on a Brand New PC

    Imaris 8.2

    Just in time for the holidays, we've upgraded our Imaris 4D rendering software and put it on a brand new, high speed PC. The new workstation is a Windows 10 platform running on dual Xeon E5-E52697v3 2.6GHz CPUs [56 virtual cores], 512GB RAM plus 512 GB fast-swap paging, M6000 nVidia graphics 12GB video RAM [3072 CUDA cores], 5k display monitor, and fast local storage disks. Software features the current version of Imaris [8.2.1] including filament tracer, cell lineage, batch, and cell modules. MATLAB 2016, Fiji, ICY, Fluorender and MS Office are also installed.





    New Equipment! Lattice Lightsheet Has Arrived!

    Posted 12/9/2015: The Lattice Lightsheet has arrived and setup has begun! Contact your favorite Technical Director for up-to-date information on build progress, expected completion date and anticipated training times.

    LLS_build_timelapse

    The UChicago Light Microscopy Core Facility will be home to a revolutionary new microscope. We have been chosen as one of 10 sites in the country to house a lattice lightsheet microscope. Invented by Nobel Laureate Dr. Eric Betzig and commercialized by 3i, this microscope allows for imaging at high spatiotemporal resoultion. You can read the original paper introducing this microscope and watch videos collected by the Betzig lab in the October 2014 issue of Science.



    Nature Article on Superresolution Features Technical Director

    UChicago Light Microscopy Core Facility Technical Director Christine Labno is quoted in the Nature feature "Super-Resolve Me: From Micro to Nano" by Michael Eisenstein. The article talks about the potential benefits and pitfalls of mass-market superresolution systems. It can be found in the October 15, 2015 issue of Nature.




    Product Demo - Vutara Single Molecule Localization Superresolution Microscope October 28-30, 2015

    The Core is happy to host Bruker's Vutara Single Molecule Localization Superresolution Microscope for three days in October. The Vutara is billed as the world's only video rate superresoution microscope and features simultaneous two color imaging (near red/ far red or green / near red). For more information or technical specs, visit theVutara website. To book a demo with your own samples, contact Vytas or Christine.




    ImageJ Summer Sessions 2015

    ImageJ Summer Sessions combine ImageJ Basics and Intermediate ImageJ into one super-sized class. Christine has scheduled three sessions for your learning pleasure:

  • Tuesday June 9th 2:00-5:00pm Cummings 119
  • Tuesday June 23rd 2:00-5:00pm Cummings 119
  • Tuesday July 14th 2:00-5:00pm Cummings 119
  • Please register by emailing Christine at ccase@uchicago.edu




    Winter and Spring Quarter 2015 ImageJ Class Dates!

    Christine has scheduled more sessions of her popular ImageJ classes: Basics, Intermediate and the Macro Writing Workshop.

    Here is the Winter and Spring quarter 2015 class dates and a description of each class.

    Our room size is limited - please register through Christine to participate.




    April 28, 2014 - Product Demo - Bruker's Opterra Multipoint Scanning Confocal Microscope

    Bruker Nano Surfaces will bring it's new Opterra Multipoint Scanning Confocal for a two week demo in KCBD 1250.

    Bruker, known for their atomic force microscopy (AFM) products, entered the world of fluorescence microscopy with its acquisition of Wisconsin-based Prairie Technologies in September 2013. The Opterra was introduced by Bruker at ASCB 2013.

    The Opterra provides high-speed confocal imaging of live samples over a range of wavelengths, and is suitable for projects where a Yokogawa or Nipkow spinning disk system would be used. For more information check out this Burker webinar. Contact Vytas Bindokas to schedule a time to bring down your samples for viewing on this exciting new instrument.




    April 24, 2014 - Scheduler Crash and Upgrade

    Sometime last night our scheduler crashed, forcing IT to upgrade us to a newer version, now called Booked. Links from all Microscopy Core website pages should now direct to the new scheduler. If not, refresh your browser and look for a "last modified" date of 4/29 or later.

    As far as we know, all 1300+ user accounts are still there, but all sign-up permissions were lost. We're working to restore everything, but there are a LOT of you. If you can't sign up for a microscope and you have been trained, contact your favorite Technical Director and we'll restore permissions. We apologize for any added stress this may cause.




    March/April 2014 - New Equipment! Perkin Elmer Pannoramic MIDI Whole Slide Scaner

    MIDI whole slide scanner
    We have added a second Pannoramic whole slide scanner thanks to funds from the Research Resources Oversight Committee (RROC).

    This scanner will run as a drop-off service in parallel with our Pannoramic SCAN whole slide scanner.

    Like the SCAN, the MIDI is capable of full color histology AND 4-color fluorescence scanning. The MIDI may only hold 12 slides per tray, but it scans at a faster rate than the SCAN, dramatically increasing our scanning output.

    Contact Shirley Bond with questions, or just stop by KCBD 1250 and have a look!




    February 2014 - New Equipment! Leica Ground State Depeletion / TIRF Microscope

    We are pleased to announce that a second superresolution microscope is available for imaging in the Core. The Leica GSDIM was provided by the Institute for Genomics & Systems Biology (IGSB) and the Institute for Molecular Engineering. It is currently housed in KCBD 1250B.

    microtubule gif
    Focal adhesions in 3D
    Image courtesy of Jiping Yue, Wu lab
    This is the same microscope we had on demo in November 2013. Thanks to all the investigators who brought samples then, it was a great demo!

    This superresolution system uses a single molecule localization or "pointilist" technique. GSD, PALM and STORM are known for their super high resolution results, achieving lateral (xy) resolution down to 20nm and axial (z) resolution down to 50nm. This represents an 8-10x improvement over confocal microscopy!

    For this technique to work, proper sample preparation is critical!

    We cannot generate images without proper sample preparation. Contact Vytas or Christine for information on sample prep and for scheduling your first session. For more information about this microscope, visit the Leica website.



    ImageJ Classes Resume for 2014

    Christine has scheduled more sessions of her popular ImageJ Basics Class, Intermediate ImageJ Class and ImageJ Macro Writing Workshops. Hooray!

    Here is the Spring 2014 schedule and a description of each class.

    Our room size is limited, so please register through Christine to participate.




    IMCF Images Featured in BioPhotonics Article

    Core Director Dr. Vytas Bindokas made a significant contribution to Live-Cell Imaging Evolves to Find New Niches by Marie Freebody in the Novemeber 2013 issue of BioPhotonics. Beautiful images collected by several Core users are featured.




    November 2013 - New Equipment! Zeiss Lightsheet Z.1 brings Single Plane Illumination Microscopy (SPIM) to the Core

    We are pleased to announce the arrival of the new Zeiss Lightsheet Z.1, brought in with funding obtained by the Institute for Genomics & Systems Biology (IGSB) and housed in KCBD 1250C.

    Zeiss Lightsheet Z.1
    Single Plane Illumination Microscopy is particularly suited to long-term timelapse imaging of larger living specimens, such as zebrafish, Drosophilia embryos, and small plants. This technology has also been used to image living and fixed 3D cell cultures, including organoids, cysts and 3D cell migration assays.

    Colors: Cyan (CFP, mCerulean); Green (GFP, YFP); Red (mCherry, DsRed).

    Magnifcations: 5x, 20x and 40x objectives with 0.4x-2.5x zoom factor.

    Environmental controls: Peltier block heating / cooling system for holding specimens at temperatures from 25-37C. Specimen chamber can be filled with PBS, nutrient buffer, or cell medium during imaging.




    November 4-6, 2013 - Leica Superresolution 3D Ground State Depletion (SR GSD 3D) Demo in KCBD 1250

    Leica Microsystems is bringing it's new 3D Ground State Depletion superresolution microscope to campus for three days only, November 4-6, 2013.

    This superresolution system uses a single molecule localization or "pointilist" technique. GSD, PALM and STORM techniques are known for their super high resolution results, achieving lateral (xy) resolution down to 20nm and axial (z) resolution down to 50nm. This represents an 8-10x improvement over confocal microscopy!

    For this technique to work, proper sample preparation is critical!

    We cannot generate images without proper sample preparation. Contact Vytas or Christine for information on sample prep and for scheduling your demo session. For more information about this microscope, visit the Leica website.




    September 2013 - New Manuscript Acknowledgment Program

    Acknowledging the Facility or the Technical Directors and listing the Core equipment you used in the Materials and Methods section helps us demonstrate how much our Core contributes to the University community and track the impact of each piece of equipment on research infrastructure.

    If you send us a .pdf copy of an accepted manuscript which acknowledges the Facility or states that you used equipment from the Facility, we will credit you ONE HOUR of FREE microscope / software time on the equipment of your choice on your next invoice. Only manuscripts accepted for publication AFTER July 2013 qualify.

    Here are sample sentences that anyone using the Facility can add to the Acknowledgements or Materials and Methods sections of appropriate manuscripts (link goes to a downloadable Word document).




    June 18 - 19, 2013 -- BioTek Cytation3 Cell Imaging / Fluorescence Plate Reader Demo

    BioTek Instruments Inc. is bringing the new Cytation3 to KCBD 1250 for a two-day demo.

    Seminar: "A New Look at Cell-Based Assays: Combining PMT-based Cell Population data with Imaging-based Sub-population Analysis"

    June 18, 2013 10:00am - 11:00am in KCBD 1103

    Demos of the Cytation during the afternoon of June 18th and morning of June 19th

    cytation flyer

     




    Equipment Improvements for Spring 2013

    We are pleased to announce valuable additions to two of our microscopes. The Leica SP5 2-photon in Abbott now boasts a 25x NA 0.95 objective for sharper, higher resolution images of large fields of view and a custom-fit, full incubation chamber for greater thermal stability during live cell imaging. The TIRF/widefield Olympus microscope in KCBD 1250 now images with a top-of-the-line Hamamatsu Orca Flash 4.0 sCMOS camera. This camera has 4.0 megapixels with a 6.5um pixel size, giving a 2.5x larger viewing area with the same pixel size / same resolution as the previous CCD camera on that system, with added speed and increased sensitivity.




    Beginning January 23, 2013 -- Olympus VS120 Virtual Slide Scanner Demo in KCBD 1250

    VS120
    The Olympus VS120 Virtual Slide System is a microscope-based whole slide scanning system capable of capturing a whole slide (with or without label) in four color fluorescence and/or full color histology.

    Tiling can be done with 2x, 10x, 20x or 40x dry objectives and then viewed digitally at virtually any magnification.

    Viewing and annotation through the free, downloadable OlyVIA software package.

    For more information, visit the Olympus product webpage.

    Contact Shirley Bond or Christine Labno (information at right) to set up an imaging session with your slides!




    Vutara SR-200 Super-Resolution Microscope Web-based Demo
    January 10, 2013 at 2:00pm in KCBD 1250

    The Light Microscopy Core will host a web-based demo of the SR-200 from the Salt Lake City-based Vutara company. This 2-color, 3-dimensional super-resolution light microscope uses point-fitting technology to achieve lateral resolution down to 20nm and axial resolution down to 50nm.




    January 4 - January 31, 2013 -- Leica TCS SP8 Demo HERE in KCBD 1250B

    SP8
    The Leica TCS SP8 STED super-resolution microscope is HERE for a demo until Thursday, January 31, 2013. The SP8 features:
    • Two HyD and three PMT detectors in an AOBS system for customizable fluorescence detection.
    • NEW tunable white light laser for excitation at any wavelength from 470nm-670nm.
    • NEW Time gating on the HyDs with gates from 3.5 nsec - 12 nsec.
    • NEW AFC focal plane drift correction system.
    • STED depletion at 592nm (single depletion line only).
    • Galvo and NEW faster resonant scanners.
    • Re-designed LCS software with new features including a brand-new 3D display module.

    More information and sample images are available on the Leica website. Contact Vytas Bindokas or Christine Labno (information at right) to set up an imaging session.




    Acknowledgement of core facility contributions in publications is appreciated.
    Cancer center members should cite the Cancer Center Support Grant (P30 CA014599) when publishing any work assisted by this core facility.