* Leica SP5 2-photon confocal
resonant galvo high-speed scanner, multiline Ar, 561, 633 lasers plus broadband, tunable Mai Tai pulsed
laser; DIC; internal APDs and two external NDDs --SPECIAL TRAINING REQUIRED--

A user-friendly, high-resolution system for confocal optical sectioning of fixed and living specimens and data analyses. The system features dual detectors for simultaneous data acquisition of green and red/far red fluorescence or DIC transmitted light images, automated optical sectioning, time lapsing, and device control (for physiological experiments).
3 ion lasers, 3 laser lines. 488nm Ar, 543, 633nm HeNe's; illumination by individual lines and 488+543 or 488+633 dual lines
dual scanning galvanometer mirrors (1.5 msec/line scan rate)
neutral density selection of 100, 50, 20, 6% laser light
5 confocal pinhole apertures
barrier filter selection for narrow or long-pass green, red, and far red (Cy5), or none for reflectance and transmitted light modes
two photomultiplier detectors, 12-bit, image resolution to 1024x1024 pixels
digital zoom 1-10X
z- stepping motor, 0.1 µm resolution (will accept piezo steppers)
scan control for x-y, xyt, xyz, xyzt, xz, and xt modes; region-of-interest scan
Tiempo physiology software (real-time intensity plots, ratio calculation, calibration, trigger in/out)
Extensive analysis capability (distance, area, intensity, multichannel, volume reconstruction, animation, tiling, math/logical, masking, digital filtering, color encoding/overlay)
Olympus IX70 (inverted) microscope with conventional fluorescence ( 100W Hg) and DIC optics
Wide GFP and "rhodamine" filter sets
100x ,60x,40x,20x,10x
PIII-450MHz PC with 512MB RAM, 68GB storage, CD, CDRW, floppy, and zip drives; ethernet and server mode.