ANALYSIS STATIONS
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Analysis Stations Computers: The available programs can automatically locate, acquire, enhance and extract objects (features) based on either color or intensity levels. Measurements of distance, area, intensity, shape and number of objects can be automatically tabulated and exported for additional statistical analysis.
- Zeiss (Mac) Software : Adobe Photoshop, Openlab (Deconvolution, Colocalization, Measurements), Slidebook, Image J, Illustrator, Adobe AfterEffects, Canvas, Toast Titanium, Microsoft Office
- Leeuwenhoek (Mac-G5) Software: Openlab (Deconvolution & Measurements, Densitiy Slicing, Colocalization), Slidebook, Illustrator, Adobe AfterEffects, Canvas, Toast Titanium, Microsoft Office
- PC Analysis Station Software: Adobe Photoshop, Image J, Metamorph, Microsoft Office
- 2 Epson 1280 Printers and Olympus P-400 Dye Sub Printer
Colocalization
T lymphocytes interacting with fibroblasts serving as antigen presenting cells. Two-color indirect immunofluorescence, FITC (green) channel: LFA-1, a T cell-specific integrin, TRITC (red) channel: talin, an actin-binding protein that interacts with the cytoplasmic tails of beta integrins, and UV (blue) channel: Hoechst Stain to stain nuclei. Yellow color results from colocalization of LFA-1 (green) and talin (red). Courtesy of C.Sedwick, J.Miller and J.Burkhardt at the University of Chicago
Above image courtesy of W. Shaalan (H. Bassiouny Lab).
The available analytical programs can automatically locate, acquire, enhance, and extract objects (features) based on either color or intensity levels. Measurements of distance, area, intensity, shape, and number of objects can automatically be tabulated and exported for additional statistical analysis.
Digital Confocal Processing (Deconvolution)
Digital confocal processing
mathematically removes the out of focus haze from the image planes above
and below the focal plane of interest. It provides an alternative methodology
to pinhole-based laser confocal microscopes. The deconvolution of a single
image or a z-series of images improves the visualization of labeled proteins
even in relatively thick specimens as demonstrated below. No special attachments
are required. Digital images captured at the imaging station are deconvolved
using the analysis station. Deconvolution does not alter the original images;
therefore, the user has the option of returning to the original data set
for additional imaging applications.
The image above is a digital deconvolution of the left image that was obtained from a z-series (Openlab dci module, Improvision, Ltd.). The C. elegans embryo is labeled for filamentous actin with Alexa-Phalloidin (Molecular Probes). Images courtesy of Vida Pratis and Judith Austin, University of Chicago. The Zeiss Axioplan microscope in the Digital Light Microscopy Facility was used.
Above Image: Raw Image/ AutoQuant processed image/ Huygens Essential processed image.
