Located in Room AB-120:

DSU Spinning disk confocal

 

 

 

 

 

Live-cell-optimized confocal inverted IX81 automated microscope changeable pinhole disks(special request) UV,green,red,cy5, CFP/GFP/YFP/DsRed Hamamatsu back-thinned EM-CCD high speed/sensitivity Slidebook4.1 and 4-D graphics

Leica SP5 2-photon confocal

 

 

 

 

 

 

 

Leica SP5 Tandem Scanner Spectral 2-Photon Confocal Microscope

Selectable slow or resonant galvanometer scanning confocal microscope is optimized for live cell imaging.Turnkey, full control via software.

1) Leica DMI6000 (inverted) microscope with conventional fluorescence (100W Hg) and DIC optics; 10x dry, 20x multi-immersion, 40x water, 63x water, and 63x oil objective.

2) 3 chilled PMT fluorescence detectors (digital spectral definition in 1 nm increments) plus one transmitted light detector. 8-, 12-, or 16-bit output.

3) 2 external nondescanned PMT detectors; 2 internal APD detectors; and green/red and CFP/YFP filters.

4) Spectra Physics Mai Tai broadband Ti:sapphire laser with 10W pump (tunable from 710 to 990 nm) (up to 2W output at 840 nm)

5) 3 visible lasers, 5 laser lines. 458, 488, 514 nm Ar, 561 (10mW) diode, and 633 nm (10mW) HeNe

6) Dual scanning galvanometer mirrors (max 16000 Hz scan rate) 25 images/sec at 512x512; strip scans to 333 fps.

7) "SuperZ" galvo focusing stage, ±1.5 mm range, "instantaneous" XZY scanning.

8) AOTF attenuation of individual visible laser lines, EOM and ND filter attenuation of NIR

9) Excitation (manual) and emission (automated) scanning capability with Linear unmixing

10) Automatically optimized confocal pinhole apertures

11) Digital zoom to 40X, line averaging to 1024, frame averaging, and frame or line accumulation

12) Transmitted detector with LWD and oil lenses suited for SHG imaging

13) FRET, deconvolution, and 3D software options

Microscope:

Conventional fluorescence filter blocks:

* I3 (blue excitation BP 450 - 490; long pass emission LP 515) typical for FITC or Cy2 etc.
* N2.1 (green excitation BP 515 - 560; long pass emission LP 590) typical for TRITC or Cy3 etc.
* Motorized coarse and fine focusing and 6-position objective turret.
* Objectives:

10x / NA 0.4 dry
20x /0.7 dry
40x / 1.2-0.75 oil
63x / 1.4-0.6 UV oil
63x / 1.3 glycerol
100x /1.40-0.7 oil

* DIC polarizer/analyzer plus prisms appropriate for each objective.
* Transmitted light detector for recording bright field images.
* Galvo stage with fast z-axis capability, and
* TMC anti-vibration table.

The following laser lines (mW power) are available: 458 (5), 476 (5), 488 (20), 514 (20), 561 (10), 633 (10) nm, fiber coupled, and controlled by AOTFs. Slow scanner with beam park,10 HZ to 1400-Hz line scans, scan resolution up to 8000x8000 pixels, zoom to 40x, scan rotation; interactive control panel with digital potentiometers.

Control: LCS Leica Confocal Software with fully operator configurable user interface. Context sensitive online help system. Multitasking high performance Windows XPpro Xeon Workstation (HP X4000) with 2.4GHz CPU, 2048MB RAM, two 21" monitors, 250 GB SATA2 hard disc, 3.5" floppy, 16x DVD writer, USB and Firewire ports. Software includes LASAF 3D Volume rendering, 3D animations, 3D filter, deconvolution, live data mode, multicolor co-localization.

Located in Room AB-129

Fluoview

A user-friendly, high-resolution system for confocal optical sectioning of fixed and living specimens and data analyses. The system features dual detectors for simultaneous data acquisition of green and red/far red fluorescence or DIC transmitted light images, automated optical sectioning, time lapsing, and device control (for physiological experiments).
3 ion lasers, 3 laser lines. 488nm Ar, 543, 633nm HeNe's; illumination by individual lines and 488+543 or 488+633 dual lines
dual scanning galvanometer mirrors (1.5 msec/line scan rate)
neutral density selection of 100, 50, 20, 6% laser light
5 confocal pinhole apertures
barrier filter selection for narrow or long-pass green, red, and far red (Cy5), or none for reflectance and transmitted light modes
two photomultiplier detectors, 12-bit, image resolution to 1024x1024 pixels
digital zoom 1-10X
z- stepping motor, 0.1 µm resolution (will accept piezo steppers)
scan control for x-y, xyt, xyz, xyzt, xz, and xt modes; region-of-interest scan
Tiempo physiology software (real-time intensity plots, ratio calculation, calibration, trigger in/out)
Extensive analysis capability (distance, area, intensity, multichannel, volume reconstruction, animation, tiling, math/logical, masking, digital filtering, color encoding/overlay)
Olympus IX70 (inverted) microscope with conventional fluorescence ( 100W Hg) and DIC optics
Wide GFP and "rhodamine" filter sets
100x ,60x,40x,20x,10x
PIII-450MHz PC with 512MB RAM, 68GB storage, CD, CDRW, floppy, and zip drives; ethernet and server mode.

Leica SP2 AOBS
spectral confocal with 405 UV laser 3CH+DIC digital emission 400-800nm range FRAP, FRET, FLIP

 

Advanced confocal system with wide range of laser excitation lines (now we can do DAPI and Texas Red) and digital definition of wavelengths captured per detector channel (total of 3). Spectral scanning and separation of similar colored fluorophores possible (e.g., GFP vs FITC or YFP).

Microscope:

DM IRE2 HCB Fluo TCS /1-B-UV Inverted microscope stand DM IRE2 for transmitted light and fluorescence. Conventional fluorescence filter blocks:

* I3 (blue excitation BP 450 -490; long pass emission LP 515) typical for FITC or Cy2 etc.
* N2.1 (green excitation BP 515 - 560; long pass emission LP 590) typical for TRITC or Cy3 etc.
* Motorized coarse and fine focusing and 6-position objective turret.
* Objectives:
o 10x / NA 0.4 dry,
o 20x /0.7 dry, 40x / 1.2-0.75 oil,
o 63x / 1.4-0.6 UV oil, 63x / 1.3 glycerol,
o 100x /1.40-0.7 oil.
* DIC polarizer/analyzer plus prisms appropriate for each objective.
* Transmitted light detector for recording bright field images.
* Galvo stage with fast z-axis capability, and
* TMC anti-vibration table.

Scanner: Scan detector equipped with an acousto-optical beam splitter (AOBS) to select/introduce most excitation laser lines (9 available), eliminating the need for main dichroic mirrors. Three PMT detectors with 8 or 12-bit output; simultaneous operation, digital/graphic definition of emission wavelength content per channel. Spectral scanning / display and linear un-mixing: the ability to separate largely overlapping spectra.

The following laser lines (mW power) are available: 405 (25), 458 (5), 476 (5), 488 (20), 496, 514 (20), 543 (1.2), 594 (orange HeNe), 633 (10) nm, fiber coupled, and controlled by AOTFs. Scanner with 2000-Hz line scans, frame scan rate of 40 fps at 512x32 pixels, scan resolution up to 4096x4096 pixels, zoom to 32x, scan rotation; interactive control panel with digital potentiometers.

Control: LCS Leica Confocal Software with fully operator configurable user interface. Context sensitive online help system. Multitasking high performance Windows XPpro Xeon Workstation (HP X4000) with 2.4GHz CPU, 2048MB RAM, two 21"CRT Monitors, 60 GB SCSI hard disc, 3.5" floppy, 16x DVD ROM, CD writer 16x/10x/40x. Software includes LCS 3D Volume rendering, 3D animations, 3D filter, Stereo images and animations, Average projection, Maximum point projection, SFP; LCS Multicolor Co-localization of 2channel and 3channel recordings. Cytofluorograms in 2D and 3D display. Histogram-segmentation and masking.