Located in Room N-710:

Zeiss Axioplan

Zeiss Axiovert 200

Zeiss Axiovert 100tv

 

 

 

 

 

 

 

 

 

 

Located in Room AB-129:

Zeiss Axiovert 135

 

 

 

 

 

 

 

 

 

 

 

 

 

Olympus IX 81

Allows for automated acquisition of high resolution image data using multiple excitation and emission wavelengths over time and space (Z axis); fluorescence and DIC.

Microscope: Inverted Olympus IX81 side port stand, Tilting binocular, Motorized DIC/Phase Condenser, U Plan Fluorite 10X Objective, NA 0.30, LWD Plan FL 20X Objective, NA 0.40 with Correction Cap for 1.1mm Thick Plastic, CAP-G0.5 Correction Cap for Glass Vessels, 40X oil NA 1.35, TIRFM-SP Plan APO 60X Oil objective; NA 1.45, U Plan APO 60X Water objective, NA 1.20, Motorized 6 position fluorescence mirror cube turret; mirror cubes: DAPI, CFP, C/YFP FRET, YFP, red, Cy5, Fura-2. High power 100W Hg arc lamp. Integral Z/focus motorization, 10nm step resolution. High resolution (1360x1036, 6.45 mm pixel), high quantum efficiency Retiga EXi digital chilled CCD camera, firewire interface, 8 or 12-bit output. Frame rates are 10 and 5 fps at full resolution (8 vs 12 bits, respectively), to 120 fps for 8-bit, 4x4-binned, 10x10 pixel subregions; exposures from 40 microsec to 15 min. Colors filters are now installed on the Retiga camera to capture color images.

Control: Intel P4 (2GHz) Windows XPpro CPU with CDRW, 80GB hard disk, running MetaMorph and MetaFluor software. Ten-position Sutter excitation and emission filter changers, Fura-2 exciters, C/YFP FRET emission filters; neutral density filters.