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The microscopes (Olympus IX81, Axiovert 100tv, the Spinning Disk, and the 2- Photon) can be equipped with a temperature controlled perfusion chambers designed to maintain cells under physiological conditions for long term observations.
The microscopes are fitted with digital cameras that enable users to follow fluorescently labeled living cells (e.g. GFP-tagged proteins) at low light levels under computer control.
Time Lapse
Spontaneous Ca Events Rodent Hippocampus.mov (Courtesy of Vytas Bindokas)
Above image file is from Ravi Palanivelu and Daphne Preuss. (2006) Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in Vitro. BMC Plant Biology 2006, 6:7
Z-stacks
Above Z-stack is from Caitlin Sedwick.
3D renderings of the plant confocal data animated gif file
from the new release of the ImageJ volume viewer plugin that now runs on RGB stacks (Courtesy of Vytas Bindokas)
Mitochondria 3D ( Courtesy of Vytas Bindokas)
